Identification of human spermatozoids in samples contaminated with yeast

Identification of human spermatozoids in samples contaminated with yeast

Sexual violence is a global problem, and represents a serious felony which is often difficult to impute due to the absence of witnesses. The evaluation of semen samples sent to the laboratory, for the detection of spermatozoa is of great importance to find the aggressor. However the evidence is not...

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Main Authors: Reina-Bouvet, Beatriz, Beatriz-Pavesi, Adriana, Vicenta-Paparella, Cecilia, María-Ombrella, Adriana
Format: Online
Language:spa
Published: Universidad Autónoma de Tamaulipas 2017
Online Access:https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/840
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id oai:ojs.pkp.sfu.ca:article-840
record_format ojs
institution CIENCIA UAT
collection OJS
language spa
format Online
author Reina-Bouvet, Beatriz
Beatriz-Pavesi, Adriana
Vicenta-Paparella, Cecilia
María-Ombrella, Adriana
spellingShingle Reina-Bouvet, Beatriz
Beatriz-Pavesi, Adriana
Vicenta-Paparella, Cecilia
María-Ombrella, Adriana
Identification of human spermatozoids in samples contaminated with yeast
author_facet Reina-Bouvet, Beatriz
Beatriz-Pavesi, Adriana
Vicenta-Paparella, Cecilia
María-Ombrella, Adriana
author_sort Reina-Bouvet, Beatriz
title Identification of human spermatozoids in samples contaminated with yeast
title_short Identification of human spermatozoids in samples contaminated with yeast
title_full Identification of human spermatozoids in samples contaminated with yeast
title_fullStr Identification of human spermatozoids in samples contaminated with yeast
title_full_unstemmed Identification of human spermatozoids in samples contaminated with yeast
title_sort identification of human spermatozoids in samples contaminated with yeast
description Sexual violence is a global problem, and represents a serious felony which is often difficult to impute due to the absence of witnesses. The evaluation of semen samples sent to the laboratory, for the detection of spermatozoa is of great importance to find the aggressor. However the evidence is not simple due to the presence of other organisms and sperm lability. The objective of  the present study was to identify human spermatozoa in samples contaminated with yeast. Different techniques and microscopic resources were applied and 46 samples of fresh semen were selected. They were divided into two groups: G1 normo or oligozoosperms (n = 28) and G2 azoosperms (n = 18). Each sample was diluted 1:1 with physiological solution and 0.05 mL of yeast suspension Candida albicans was added. As controls, pure sperm (CE) and pure yeast (CL) were processed. The samples were analyzed with optical microscopy (MO), phase contrast (CF), polarized light (LP) and fluorescent microscope (MF) with different staining techniques. Papanicolaou dye showed greater efficiency than bright hematoxylin-green dye (P < 0.01) to identify spermatozoa with an optical microscope. The presence of refringent elements showed a higher correlation with the presence of spermatozoa (LP: r = 0.966; P < 0.001),  that the presence of birrefringent elements (LP: r = 0.737; P < 0.001) and fluorescent elements (MF: r = 0.487, P < 0.05). Confocal microscopy with Evans blue calcofluor white stain and acridine orange allowed the identification of yeast contamination. The use of microscopic techniques aided by staining techniques confirms the presence of whole or fragmented spermatozoa in semen samples contaminated with yeasts
publisher Universidad Autónoma de Tamaulipas
publishDate 2017
url https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/840
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spelling oai:ojs.pkp.sfu.ca:article-8402019-10-24T12:18:01Z Identification of human spermatozoids in samples contaminated with yeast Identificación de espermatozoides humanos en muestras contaminadas con levaduras Reina-Bouvet, Beatriz Beatriz-Pavesi, Adriana Vicenta-Paparella, Cecilia María-Ombrella, Adriana spermatozoa yeast semen forensic medicine microscopic techniques. espermatozoide levaduras semen medicina legal técnicas microscópicas. Sexual violence is a global problem, and represents a serious felony which is often difficult to impute due to the absence of witnesses. The evaluation of semen samples sent to the laboratory, for the detection of spermatozoa is of great importance to find the aggressor. However the evidence is not simple due to the presence of other organisms and sperm lability. The objective of  the present study was to identify human spermatozoa in samples contaminated with yeast. Different techniques and microscopic resources were applied and 46 samples of fresh semen were selected. They were divided into two groups: G1 normo or oligozoosperms (n = 28) and G2 azoosperms (n = 18). Each sample was diluted 1:1 with physiological solution and 0.05 mL of yeast suspension Candida albicans was added. As controls, pure sperm (CE) and pure yeast (CL) were processed. The samples were analyzed with optical microscopy (MO), phase contrast (CF), polarized light (LP) and fluorescent microscope (MF) with different staining techniques. Papanicolaou dye showed greater efficiency than bright hematoxylin-green dye (P < 0.01) to identify spermatozoa with an optical microscope. The presence of refringent elements showed a higher correlation with the presence of spermatozoa (LP: r = 0.966; P < 0.001),  that the presence of birrefringent elements (LP: r = 0.737; P < 0.001) and fluorescent elements (MF: r = 0.487, P < 0.05). Confocal microscopy with Evans blue calcofluor white stain and acridine orange allowed the identification of yeast contamination. The use of microscopic techniques aided by staining techniques confirms the presence of whole or fragmented spermatozoa in semen samples contaminated with yeasts La violencia sexual es un problema mundial, y representa un delito grave que frecuentemente es difícil imputar, ante la ausencia de testigos. La valoración de las muestras de semen enviadas al laboratorio, para la detección de espermatozoides, es de gran importancia para encontrar al agresor; sin embargo, la evidencia no es sencilla, por la presencia de otros organismos y la labilidad espermática. El objetivo del presente estudio fue identificar espermatozoides humanos en muestras contaminadas con levaduras. Se aplicaron diferentes técnicas y recursos microscópicos. Se seleccionaron 46 muestras de semen fresco. Se dividieron en dos grupos: G1 normo u oligozoospérmicas (n = 28) y G2 azoospérmicas (n = 18). Cada muestra se diluyó 1:1 con solución fisiológica y se agregaron 0.05 mL de suspensión de levaduras Candida albicans. Como control, se procesaron testigos puros de espermato-zoides (CE) y levaduras (CL). Las muestras se analizaron con microscopio óptico (MO), contraste de fase (CF), de luz polarizada (LP) y microscopio fluorescente (MF) con diferentes técnicas de tinción. El tinte Papanicolaou mostró mayor eficiencia que el tinte hematoxilina verde brillante (P < 0.05) para identificar espermatozoides con microscopio óptico. La detección de elementos refringentes mostró mayor correlación con la presencia de espermatozoides (CF: r = 0.966; P < 0.001), que la presencia de elementos birrefringentes (LP: r = 0.737; P < 0.001) y elementos fluorescentes (MF: r = 0.487; P < 0.05). La microscopía confocal con tinción de blanco de calcofluor-azul de Evans y naranja de acridina permitió la identificación de contaminación por levaduras. El empleo de técnicas microscópicas auxiliadas con técnicas de tinción permite confirmar la presencia de espermatozoides enteros o fragmentados en muestras de semen contaminadas con levaduras. Universidad Autónoma de Tamaulipas 2017-07-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html application/xml https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/840 10.29059/cienciauat.v12i1.840 CienciaUAT; Vol. 12 No. 1: July-December 2017; 23-35 CienciaUAT; Vol. 12 No. 1: Julio-Diciembre 2017; 23-35 2007-7858 2007-7521 spa https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/840/414 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/840/484 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/840/627 Derechos de autor 2017 CienciaUAT

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