Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken

With the discovery of antibiotics, millions of lives have been saved, however, its effectiveness has been declining has been declining at the same microorganisms are developing resistance because of their constant exposure during application on the growth of animals for slaugther, so it is necessary...

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Main Authors:	Monroy-Torres, Rebeca, Linares-Segovia, Benigno, Ramírez-Gómez, Xochitl Sofía
Format:	Online
Language:	spa
Published:	Universidad Autónoma de Tamaulipas 2015
Online Access:	https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/738
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spelling	oai:ojs.pkp.sfu.ca:article-7382019-03-22T11:02:45Z Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken Desarrollo de una técnica para la detección in vitro de la presencia de antibióticos en muestras de hígado de res, cerdo y pollo Monroy-Torres, Rebeca Linares-Segovia, Benigno Ramírez-Gómez, Xochitl Sofía Antibiotics Escherichia coli liver inhibitory effect. Antibióticos Escherichia coli hígado efecto inhibitorio. With the discovery of antibiotics, millions of lives have been saved, however, its effectiveness has been declining has been declining at the same microorganisms are developing resistance because of their constant exposure during application on the growth of animals for slaugther, so it is necessary to have techniques for the early detection of their presence in food. A cross-sectional and preliminar study was conducted to stablish the feasibility to determine the presence of antibiotics in samples of liver of beef, pork and chicken by inhibiting the growing of the Escherichia coli ATCC25922 strain following the Kirby-Bauer methodology. Fiftheen Petri plates (five for each liver sample: beef, pork and poultry) containing the Mueller Hinton agar were inoculated with the E. coliATCC25922 strain antibiotic sensitive. In each agar plate a portion of liver of 5 mm of diameter were placed 1 cm away from each inoculum. The plates were incubated at 35 OC for 24 h and the presence of inhibition halos were evaluated. It was found that only one of the fiftheen liver samples analyzed, corresponding to a beef liver, showed a inhibition halo higher than 30 mm. The results obtained indicated the feasibility of using this technique to detect the presence of antibiotics in meat products.Archive XML (SciELO) El descubrimiento de los antibióticos ha permitido salvar la vida de millones de personas, sin embargo, su efectividad ha ido disminuyendo a la par que los microorganismos van desarrollando resistencia debido a su exposición constante durante su aplicación en el crecimiento de animales de abasto, por lo que se hace necesario contar con técnicas de detección oportuna de su presencia en alimentos. Se realizó un estudio preliminar, de corte transversal, en muestras de hígado de res, cerdo y pollo para establecer la viabilidad de determinar la presencia de antibióticos mediante la inhibición del crecimiento de una cepa de Escherichia coli ATCC25922, utilizando el método de Kirby-Bauer. Se inocularon 15 cajas Petri conteniendo agar Mueller Hinton (cinco para cada muestra de hígado: res, cerdo y pollo), con la cepa E. Coli sensible a antibióticos. En cada caja de agar se colocaron porciones de hígado de 5 mm de diámetro, con 1 cm de distancia entre muestras. Las cajas se incubaron a 35 OC por 24 h y se evaluó la formación de halos de inhibición. Se encontró que sólo una de las 15 muestras de hígado analizadas, correspondiente a hígado de res, presentó un halo de inhibición mayor a 30 mm. Los resultados obtenidos indican la viabilidad de usar esta técnica para la detección de la presencia de antibióticos en productos cárnicos.Archivo XML (SciELO) Universidad Autónoma de Tamaulipas 2015-07-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html application/xml https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/738 10.29059/cienciauat.v9i2.738 CienciaUAT; Vol. 9 No. 2: January-June 2015; 68-73 CienciaUAT; Vol. 9 No. 2: Enero-Junio 2015; 68-73 2007-7858 2007-7521 spa https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/738/357 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/738/540 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/738/573 Derechos de autor 2015 CienciaUAT
institution	CIENCIA UAT
collection	OJS
language	spa
format	Online
author	Monroy-Torres, Rebeca Linares-Segovia, Benigno Ramírez-Gómez, Xochitl Sofía
spellingShingle	Monroy-Torres, Rebeca Linares-Segovia, Benigno Ramírez-Gómez, Xochitl Sofía Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
author_facet	Monroy-Torres, Rebeca Linares-Segovia, Benigno Ramírez-Gómez, Xochitl Sofía
author_sort	Monroy-Torres, Rebeca
title	Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
title_short	Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
title_full	Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
title_fullStr	Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
title_full_unstemmed	Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
title_sort	development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken
description	With the discovery of antibiotics, millions of lives have been saved, however, its effectiveness has been declining has been declining at the same microorganisms are developing resistance because of their constant exposure during application on the growth of animals for slaugther, so it is necessary to have techniques for the early detection of their presence in food. A cross-sectional and preliminar study was conducted to stablish the feasibility to determine the presence of antibiotics in samples of liver of beef, pork and chicken by inhibiting the growing of the Escherichia coli ATCC25922 strain following the Kirby-Bauer methodology. Fiftheen Petri plates (five for each liver sample: beef, pork and poultry) containing the Mueller Hinton agar were inoculated with the E. coliATCC25922 strain antibiotic sensitive. In each agar plate a portion of liver of 5 mm of diameter were placed 1 cm away from each inoculum. The plates were incubated at 35 OC for 24 h and the presence of inhibition halos were evaluated. It was found that only one of the fiftheen liver samples analyzed, corresponding to a beef liver, showed a inhibition halo higher than 30 mm. The results obtained indicated the feasibility of using this technique to detect the presence of antibiotics in meat products.Archive XML (SciELO)
publisher	Universidad Autónoma de Tamaulipas
publishDate	2015
url	https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/738
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Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken

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