Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico

Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico

Human listeriosis was an uncommon disease until few years ago. In the 1980s it became an emerging food-borne disease. Human listeriosis could be found as isolated cases or in outbreaks related to contaminated foods like raw vegetables, milk, fresh cheese and raw or poorly cooked beef and poultry pro...

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Main Authors: Cruz-Pulido, Wendy Lizeth, Téllez-Luis, Simón Josías, Rivera-Sánchez, Gildardo, Ávila-Aguilar, Selene, Cantú-Ramírez, Rubén, Garza-González, Elvira, Sierra-Juárez, Graciela, Bocanegra-García, Virgilio
Format: Online
Language:eng
Published: Universidad Autónoma de Tamaulipas 2012
Online Access:https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/51
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spelling oai:ojs.pkp.sfu.ca:article-512015-09-30T10:10:52Z Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico Listeria sp. y Listeria monocytogenes en pollo congelado: detección por NOM-143-SSA1-1995 y PCR de expendios comerciales de Matamoros y Reynosa, Tamaulipas, México Cruz-Pulido, Wendy Lizeth Téllez-Luis, Simón Josías Rivera-Sánchez, Gildardo Ávila-Aguilar, Selene Cantú-Ramírez, Rubén Garza-González, Elvira Sierra-Juárez, Graciela Bocanegra-García, Virgilio human listeriosis molecular epidemiology poultry Listeria monocytogenes. listeriosis epidemiología molecular pollo crudo Listeria monocytogenes. Human listeriosis was an uncommon disease until few years ago. In the 1980s it became an emerging food-borne disease. Human listeriosis could be found as isolated cases or in outbreaks related to contaminated foods like raw vegetables, milk, fresh cheese and raw or poorly cooked beef and poultry products. The aim of this work was to determinate the prevalence of Listeria sp. and Listeria monocytogenes in poultry samples using the Mexican standar method NOM-143- SSA1-1995 and PCR detection based on the detection of rRNA 16s for Listeria sp. and listeriolisin O gen hlyA for L. monocytogenes. Using NOM-143-SSA1-1995 standar methods we detected 14.2% of Listeria sp. and any L. monocytogenes was detected. With the molecular Listeria sp. was detected in 17.1% and L. monocytogenes was detected in 1.4% of poultry samples. These results are according with previous reports in scientific literature and in the case of L. monocytogenes our detected prevalence is below the range detected in other international reports. La listeriosis humana había sido hasta hace pocos años una enfermedad poco común. A partir de la década de 1980 empezó a manifestarse como una enfermedad de transmisión alimentaria emergente. La listeriosis humana puede presentarse en casos aislados o en brotes asociados al consumo de alimentos contaminados, como vegetales crudos, leche, quesos frescos y productos cárnicos crudos o insuficientemente cocidos. El objetivo del presente estudio fue determinar la prevalencia de Listeria sp. y L. monocytogenes en muestras de pollo crudo utilizando dos métodos de diagnóstico: la NOM-143-SSA1-1995 y la técnica de PCR basada en la detección molecular de la región RNAr 16S para Listeria sp. y el gen hlyA de la listeriolosina O para L. monocytogenes. Mediante la norma oficial mexicana NOM-143-SSA1-1995 se detectó una prevalencia de 14.2 % de Listeria sp. y no se detectó L. monocytogenes en ninguna muestra. Mediante PCR se detectó 17.1 % de prevalencia para Listeria sp. y 1.4 % para L. monocytogenes. Estas prevalencias se encuentran dentro del rango reportado en trabajos anteriores, excepto la detectada para L. monocytogenes, que se encuentra por debajo de los rangos reportados. Universidad Autónoma de Tamaulipas 2012-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/51 10.29059/cienciauat.v6i3.51 CienciaUAT; Vol. 6 No. 3: January-June 2012; 41-47 CienciaUAT; Vol. 6 No. 3: Enero-Junio 2012; 41-47 2007-7858 2007-7521 eng https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/51/41
institution CIENCIA UAT
collection OJS
language eng
format Online
author Cruz-Pulido, Wendy Lizeth
Téllez-Luis, Simón Josías
Rivera-Sánchez, Gildardo
Ávila-Aguilar, Selene
Cantú-Ramírez, Rubén
Garza-González, Elvira
Sierra-Juárez, Graciela
Bocanegra-García, Virgilio
spellingShingle Cruz-Pulido, Wendy Lizeth
Téllez-Luis, Simón Josías
Rivera-Sánchez, Gildardo
Ávila-Aguilar, Selene
Cantú-Ramírez, Rubén
Garza-González, Elvira
Sierra-Juárez, Graciela
Bocanegra-García, Virgilio
Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico
author_facet Cruz-Pulido, Wendy Lizeth
Téllez-Luis, Simón Josías
Rivera-Sánchez, Gildardo
Ávila-Aguilar, Selene
Cantú-Ramírez, Rubén
Garza-González, Elvira
Sierra-Juárez, Graciela
Bocanegra-García, Virgilio
author_sort Cruz-Pulido, Wendy Lizeth
title Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico
title_short Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico
title_full Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico
title_fullStr Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico
title_full_unstemmed Listeria sp. and Listeria monocytogenes in frozen poultry: Detection by NOM-143-SSA1-1995 and PCR from retail stores from Matamoros and Reynosa, Tamaulipas, Mexico
title_sort listeria sp. and listeria monocytogenes in frozen poultry: detection by nom-143-ssa1-1995 and pcr from retail stores from matamoros and reynosa, tamaulipas, mexico
description Human listeriosis was an uncommon disease until few years ago. In the 1980s it became an emerging food-borne disease. Human listeriosis could be found as isolated cases or in outbreaks related to contaminated foods like raw vegetables, milk, fresh cheese and raw or poorly cooked beef and poultry products. The aim of this work was to determinate the prevalence of Listeria sp. and Listeria monocytogenes in poultry samples using the Mexican standar method NOM-143- SSA1-1995 and PCR detection based on the detection of rRNA 16s for Listeria sp. and listeriolisin O gen hlyA for L. monocytogenes. Using NOM-143-SSA1-1995 standar methods we detected 14.2% of Listeria sp. and any L. monocytogenes was detected. With the molecular Listeria sp. was detected in 17.1% and L. monocytogenes was detected in 1.4% of poultry samples. These results are according with previous reports in scientific literature and in the case of L. monocytogenes our detected prevalence is below the range detected in other international reports.
publisher Universidad Autónoma de Tamaulipas
publishDate 2012
url https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/51
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