Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves

Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves

Conventional phytochemicals extraction methods have shown low yields or very long processing times for obtaining. The ultrasound offers alternatives to increase the yields of plant extracts. The objective of this work was to evaluate the effect of three extraction methods on the yield, phenolic comp...

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Main Authors: Malpica-Acosta, Sheila Briget, Acosta-Osorio, Andrés Antonio, Benedito-Fort, José Javier, Castillo-Zamudio, Rosa Isela
Format: Online
Language:spa
Published: Universidad Autónoma de Tamaulipas 2024
Online Access:https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1797
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id oai:ojs.pkp.sfu.ca:article-1797
record_format ojs
institution CIENCIA UAT
collection OJS
language spa
format Online
author Malpica-Acosta, Sheila Briget
Acosta-Osorio, Andrés Antonio
Benedito-Fort, José Javier
Castillo-Zamudio, Rosa Isela
spellingShingle Malpica-Acosta, Sheila Briget
Acosta-Osorio, Andrés Antonio
Benedito-Fort, José Javier
Castillo-Zamudio, Rosa Isela
Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
author_facet Malpica-Acosta, Sheila Briget
Acosta-Osorio, Andrés Antonio
Benedito-Fort, José Javier
Castillo-Zamudio, Rosa Isela
author_sort Malpica-Acosta, Sheila Briget
title Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
title_short Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
title_full Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
title_fullStr Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
title_full_unstemmed Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
title_sort effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from plectranthus amboinicus leaves
description Conventional phytochemicals extraction methods have shown low yields or very long processing times for obtaining. The ultrasound offers alternatives to increase the yields of plant extracts. The objective of this work was to evaluate the effect of three extraction methods on the yield, phenolic compound composition, antioxidant activity and stability of Plectranthus amboinicus (oregano) leaf extracts. The extraction methods used were: conventional heat-assisted extraction (CE), ultrasound water bath assisted (UBAE) and ultrasound probe assisted extraction (UPAE). A 1:10 (w/v) oregano: ethanol ratio was used at 35 ± 2 °C. Yields were calculated as extract dry matter (% ms). A pseudo-second-order model was used to fit the experimental extraction curves of the three methods and obtain the kinetic parameters. Total phenolic compounds (TPC) and antioxidant activity (AA) were determined by Folin-Ciocalteau and DPPH methods, respectively. The stability of TPC and AA was evaluated during storage. UPAE increased 33.93 % yield in extracts concerning CE. The highest concentration of CFT (P ≤ 0.05) was obtained by UBAE (10.32 mg EAG/ms) and for AA by CE (19.43 mg ET/g ms). The pseudo-second-order kinetic model adequately fitted the experimental kinetics. The highest TPC rate constant (k) was 0.016 g ms/mg EAG·min, for UPAE, this technique being the one that demonstrated a significant decrease in AA with respect to extraction time. The TPC of the extracts obtained by UBAE, UPAE and CE (3.7 mg EAG/g dms, 4.4 mg EAG/g dms and 7.2 mg EAG/g ms, respectively), as well as the AA remained stable and without significant changes (P ≥ 0.05) during the 34 d of storage, again showing the advantage of using the ultrasound extractive technique, as a function of time.
publisher Universidad Autónoma de Tamaulipas
publishDate 2024
url https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1797
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spelling oai:ojs.pkp.sfu.ca:article-17972024-03-22T16:31:19Z Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves Efecto de tres métodos de extracción en el rendimiento, actividad antioxidante, fenoles totales y estabilidad de extractos de hojas de Plectranthus amboinicus Malpica-Acosta, Sheila Briget Acosta-Osorio, Andrés Antonio Benedito-Fort, José Javier Castillo-Zamudio, Rosa Isela Oreganón extracción actividad antioxidante compuestos fenólicos rendimiento Oreganon extraction antioxidant activity phenolic compounds yield Conventional phytochemicals extraction methods have shown low yields or very long processing times for obtaining. The ultrasound offers alternatives to increase the yields of plant extracts. The objective of this work was to evaluate the effect of three extraction methods on the yield, phenolic compound composition, antioxidant activity and stability of Plectranthus amboinicus (oregano) leaf extracts. The extraction methods used were: conventional heat-assisted extraction (CE), ultrasound water bath assisted (UBAE) and ultrasound probe assisted extraction (UPAE). A 1:10 (w/v) oregano: ethanol ratio was used at 35 ± 2 °C. Yields were calculated as extract dry matter (% ms). A pseudo-second-order model was used to fit the experimental extraction curves of the three methods and obtain the kinetic parameters. Total phenolic compounds (TPC) and antioxidant activity (AA) were determined by Folin-Ciocalteau and DPPH methods, respectively. The stability of TPC and AA was evaluated during storage. UPAE increased 33.93 % yield in extracts concerning CE. The highest concentration of CFT (P ≤ 0.05) was obtained by UBAE (10.32 mg EAG/ms) and for AA by CE (19.43 mg ET/g ms). The pseudo-second-order kinetic model adequately fitted the experimental kinetics. The highest TPC rate constant (k) was 0.016 g ms/mg EAG·min, for UPAE, this technique being the one that demonstrated a significant decrease in AA with respect to extraction time. The TPC of the extracts obtained by UBAE, UPAE and CE (3.7 mg EAG/g dms, 4.4 mg EAG/g dms and 7.2 mg EAG/g ms, respectively), as well as the AA remained stable and without significant changes (P ≥ 0.05) during the 34 d of storage, again showing the advantage of using the ultrasound extractive technique, as a function of time. Los métodos de extracción de fitoquímicos convencionales presentan bajos rendimientos o lar-gos tiempos de proceso. El ultrasonido ofrece alternativas para incrementar el rendimiento en extractos vegetales. El objetivo de este trabajo fue evaluar el efecto de tres métodos de extracción en rendimiento, composición de compuestos fenólicos, actividad antioxidante y estabilidad de extractos de hojas de Plectranthus amboinicus (oreganón). Los métodos de extracción usados fueron: convencional con agitación (EC), asistida con baño de ultrasonidos (EABU) y asistida con sonda de ultrasonido (EASU). Se usó una proporción 1:10 (p/v) orégano: etanol a 35 ± 2 °C. Los rendimientos se calcularon como materia seca del extracto (% ms). Se usó un modelo de pseudo-segundo orden para ajustar las curvas de extracción experimentales de los tres métodos y obtener los parámetros cinéticos. Se determinaron compuestos fenólicos totales (CFT) y actividad antioxidante (AA) mediante los métodos de Folin-Ciocalteau y DPPH, respectivamente. Se evalúo la estabilidad del CFT y AA durante el almacenamiento. La EASU aumentó 33.93 % el rendimiento en los extractos con res-pecto a EC. La mayor concentración de CFT (P ≤ 0.05) se obtuvo mediante EABU (10.32 mg EAG/ms) y para AA mediante EC (19.43 mg ET/g ms). El modelo cinético de pseudo-segundo orden ajustó adecuadamente las cinéticas experimentales. La constante de velocidad (k) de CFT más alta fue de 0.016 g ms/mg EAG·min para la EASU, siendo esta técnica la que demostró una disminución significativa de AA con respecto al tiempo de extracción. Los CFT de los extractos obtenidos mediante EABU, EASU y EC (3.7 mg EAG/g ms, 4.4 mg EAG/g ms y 7.2 mg EAG/g ms, respectivamente), al igual que la AA se mantuvieron estables y sin cambios significativos (P ≥ 0.05) durante los 34 d de almacenamiento, mostrando nuevamente la ventaja de utilizar la técnica extractiva de ultrasonidos, en función del tiempo. Universidad Autónoma de Tamaulipas 2024-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html text/xml https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1797 10.29059/cienciauat.v18i2.1797 CienciaUAT; Vol 18 No. 2. January-June 2024; 91-106 CienciaUAT; Vol. 18 No. 2: Enero-Junio 2024; 91-106 2007-7858 2007-7521 spa https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1797/1182 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1797/1166 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1797/1212 Derechos de autor 2023 Universidad Autónoma de Tamaulipas https://creativecommons.org/licenses/by-nc-sa/4.0

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