Environmental DNA metabarcoding: an approach for biodiversity monitoring

Environmental DNA metabarcoding: an approach for biodiversity monitoring

Environmental deoxyribonucleic acid or environmental DNA (eDNA) is a term coined to define the deoxyribonucleic acid (DNA) that can be recovered or detected in an environment (for example: soil, air, or water) sample, but not specimen physically present (metagenome). The objective of this review was...

Full description

Saved in:
Bibliographic Details
Main Authors: Padilla-García, Cinthia Yedith, Camacho-Sánchez, Fátima Yedith, Reyes-López, Miguel Ángel
Format: Online
Language:spa
Published: Universidad Autónoma de Tamaulipas 2021
Online Access:https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1509
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:ojs.pkp.sfu.ca:article-1509
record_format ojs
spelling oai:ojs.pkp.sfu.ca:article-15092021-07-30T21:35:44Z Environmental DNA metabarcoding: an approach for biodiversity monitoring Metabarcoding de DNA ambiental: un enfoque para el seguimiento de la biodiversidad Padilla-García, Cinthia Yedith Camacho-Sánchez, Fátima Yedith Reyes-López, Miguel Ángel eDNA biodiversity PCR sequencing eDNA biodiversidad PCR secuenciación Environmental deoxyribonucleic acid or environmental DNA (eDNA) is a term coined to define the deoxyribonucleic acid (DNA) that can be recovered or detected in an environment (for example: soil, air, or water) sample, but not specimen physically present (metagenome). The objective of this review was to understand, analyze, and define the uses, applications, and potential of eDNA. eDNA brings together several fields ranging from ecological assessment for historical community reconstruction, ecosystem restoration to human health, making it extremely versatile and important for the future in research, such as studies of conservation, taxonomic, or phylogenetic reconstruction. To achieve this, the metabarcoding procedure is used, which is based on obtaining DNA of any origin (in this case eDNA), in the physical absence or not of organisms, with the polymerase chain reaction (PCR), to finally sequence them and develop barcodes. The application of eDNA in biological research seems almost limitless, but it requires collaboration and coordination of scientific teams. eDNA studies will probably become an essential tool for different scientific tasks not only in monitoring biodiversity, but also in human health analysis or in the development of DNA barcodes. El término ácido desoxirribonucleico ambiental o DNA ambiental (eDNA) se acuñó para definir al ácido desoxirribonucleico (DNA) que se puede recuperar o detectar del ambiente (por ejemplo: suelo, aire o agua) sin necesidad de que el espécimen esté físicamente presente. El objetivo del presente trabajo fue analizar y ejemplificar los usos, aplicaciones y potencial del eDNA. El estudio del eDNA es utilizado para la evaluación de especies, que va desde la reconstrucción histórica de sus comunidades, la restauración del ecosistema, hasta la salud humana, lo que lo convierte en una herramienta versátil e importante para el futuro en investigación, permitiendo estudios de conservación, taxonómicos o de reconstrucción filogenéticos. Para lograr esto, se usa el procedimiento de metabarcoding, el cual se basa en obtener DNA de cualquier origen (en este caso eDNA), en ausencia física o no del organismo, con apoyo de la reacción en cadena de la polimerasa (PCR), para finalmente, secuenciarlos y obtener códigos de barras. Los estudios de eDNA probablemente se constituirán como un enfoque esencial para diversas tareas científicas no solo en el seguimiento de la biodiversidad, sino en el análisis de la salud humana o la generación de códigos de barras de DNA.   Universidad Autónoma de Tamaulipas 2021-07-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html text/xml https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1509 10.29059/cienciauat.v16i1.1509 CienciaUAT; Vol. 16 No. 1. July-December 2021; 136-149 CienciaUAT; Vol. 16 No. 1: Julio-Diciembre 2021; 136-149 2007-7858 2007-7521 spa https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1509/856 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1509/873 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1509/890 Derechos de autor 2021 Universidad Autónoma de Tamaulipas https://creativecommons.org/licenses/by-nc-sa/4.0
institution CIENCIA UAT
collection OJS
language spa
format Online
author Padilla-García, Cinthia Yedith
Camacho-Sánchez, Fátima Yedith
Reyes-López, Miguel Ángel
spellingShingle Padilla-García, Cinthia Yedith
Camacho-Sánchez, Fátima Yedith
Reyes-López, Miguel Ángel
Environmental DNA metabarcoding: an approach for biodiversity monitoring
author_facet Padilla-García, Cinthia Yedith
Camacho-Sánchez, Fátima Yedith
Reyes-López, Miguel Ángel
author_sort Padilla-García, Cinthia Yedith
title Environmental DNA metabarcoding: an approach for biodiversity monitoring
title_short Environmental DNA metabarcoding: an approach for biodiversity monitoring
title_full Environmental DNA metabarcoding: an approach for biodiversity monitoring
title_fullStr Environmental DNA metabarcoding: an approach for biodiversity monitoring
title_full_unstemmed Environmental DNA metabarcoding: an approach for biodiversity monitoring
title_sort environmental dna metabarcoding: an approach for biodiversity monitoring
description Environmental deoxyribonucleic acid or environmental DNA (eDNA) is a term coined to define the deoxyribonucleic acid (DNA) that can be recovered or detected in an environment (for example: soil, air, or water) sample, but not specimen physically present (metagenome). The objective of this review was to understand, analyze, and define the uses, applications, and potential of eDNA. eDNA brings together several fields ranging from ecological assessment for historical community reconstruction, ecosystem restoration to human health, making it extremely versatile and important for the future in research, such as studies of conservation, taxonomic, or phylogenetic reconstruction. To achieve this, the metabarcoding procedure is used, which is based on obtaining DNA of any origin (in this case eDNA), in the physical absence or not of organisms, with the polymerase chain reaction (PCR), to finally sequence them and develop barcodes. The application of eDNA in biological research seems almost limitless, but it requires collaboration and coordination of scientific teams. eDNA studies will probably become an essential tool for different scientific tasks not only in monitoring biodiversity, but also in human health analysis or in the development of DNA barcodes.
publisher Universidad Autónoma de Tamaulipas
publishDate 2021
url https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1509
work_keys_str_mv AT padillagarciacinthiayedith environmentaldnametabarcodinganapproachforbiodiversitymonitoring
AT camachosanchezfatimayedith environmentaldnametabarcodinganapproachforbiodiversitymonitoring
AT reyeslopezmiguelangel environmentaldnametabarcodinganapproachforbiodiversitymonitoring
AT padillagarciacinthiayedith metabarcodingdednaambientalunenfoqueparaelseguimientodelabiodiversidad
AT camachosanchezfatimayedith metabarcodingdednaambientalunenfoqueparaelseguimientodelabiodiversidad
AT reyeslopezmiguelangel metabarcodingdednaambientalunenfoqueparaelseguimientodelabiodiversidad
_version_ 1779057843818725376

Similar Items