Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla

Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla

of reproductive age. The diagnosis can be made by applying clinical criteria or by the eva-luation of bacterial morphotypes present in Gram stain performed to vaginal secretion or by microbiological procedures, which were developed as an alternative to clinical diagnosis, gradually replacing it. The...

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Main Authors: Jiménez-Flores, Guadalupe, Flores-Tlalpa, Jesús, Ruiz-Tagle, Alejandro César, Villagrán-Padilla, Claudy Lorena
Format: Online
Language:spa
Published: Universidad Autónoma de Tamaulipas 2020
Online Access:https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1296
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id oai:ojs.pkp.sfu.ca:article-1296
record_format ojs
institution CIENCIA UAT
collection OJS
language spa
format Online
author Jiménez-Flores, Guadalupe
Flores-Tlalpa, Jesús
Ruiz-Tagle, Alejandro César
Villagrán-Padilla, Claudy Lorena
spellingShingle Jiménez-Flores, Guadalupe
Flores-Tlalpa, Jesús
Ruiz-Tagle, Alejandro César
Villagrán-Padilla, Claudy Lorena
Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla
author_facet Jiménez-Flores, Guadalupe
Flores-Tlalpa, Jesús
Ruiz-Tagle, Alejandro César
Villagrán-Padilla, Claudy Lorena
author_sort Jiménez-Flores, Guadalupe
title Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla
title_short Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla
title_full Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla
title_fullStr Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla
title_full_unstemmed Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla
title_sort evaluation of methods used for the diagnosis of bacterial vaginosis in the hospital regional issste puebla
description of reproductive age. The diagnosis can be made by applying clinical criteria or by the eva-luation of bacterial morphotypes present in Gram stain performed to vaginal secretion or by microbiological procedures, which were developed as an alternative to clinical diagnosis, gradually replacing it. The objective of this work was to determine the effectiveness of the Amsel and Ison-Hay methods, used for the diagnosis of bacterial vaginosis using the Nugent method as a standard. In this study, 305 samples of vaginal discharge from patients of the ISSSTE Puebla Regional Hospital were analyzed. The samples were processed and analyzed for the diagnosis of BV following the recommendations of the Amsel, Nugent and Ison-Hay methods. The analysis of the results indicated 12.8 % by Nugent, 31.1 % of BV by the Amsel method and 36.7 % by Ison-Hay; suggesting that both techniques show a high rate of false positives. The sensitivity for the Amsel and Ison-Hay method was 97.44 %, the specificity was 78.57 % and 72.18 % for Amsel and Ison-Hay, respectively. In conclusion, given the results obtained and the minimal differences between the analyzed methods, it is recommended to perform the Amsel criteria, followed by the observation of Gram staining of the vaginal flow for the assessment of the microbiota by the Ison-Hay method, to have a better diagnosis of BV, when the Nugent method is not used.
publisher Universidad Autónoma de Tamaulipas
publishDate 2020
url https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1296
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spelling oai:ojs.pkp.sfu.ca:article-12962020-07-31T23:34:40Z Evaluation of methods used for the diagnosis of bacterial vaginosis in the Hospital Regional ISSSTE Puebla Evaluación de los métodos utilizados para el diagnóstico de vaginosis bacteriana en el Hospital Regional ISSSTE Puebla Jiménez-Flores, Guadalupe Flores-Tlalpa, Jesús Ruiz-Tagle, Alejandro César Villagrán-Padilla, Claudy Lorena bacterial vaginosis sensitivity specificity vaginosis bacteriana especificidad sensibilidad of reproductive age. The diagnosis can be made by applying clinical criteria or by the eva-luation of bacterial morphotypes present in Gram stain performed to vaginal secretion or by microbiological procedures, which were developed as an alternative to clinical diagnosis, gradually replacing it. The objective of this work was to determine the effectiveness of the Amsel and Ison-Hay methods, used for the diagnosis of bacterial vaginosis using the Nugent method as a standard. In this study, 305 samples of vaginal discharge from patients of the ISSSTE Puebla Regional Hospital were analyzed. The samples were processed and analyzed for the diagnosis of BV following the recommendations of the Amsel, Nugent and Ison-Hay methods. The analysis of the results indicated 12.8 % by Nugent, 31.1 % of BV by the Amsel method and 36.7 % by Ison-Hay; suggesting that both techniques show a high rate of false positives. The sensitivity for the Amsel and Ison-Hay method was 97.44 %, the specificity was 78.57 % and 72.18 % for Amsel and Ison-Hay, respectively. In conclusion, given the results obtained and the minimal differences between the analyzed methods, it is recommended to perform the Amsel criteria, followed by the observation of Gram staining of the vaginal flow for the assessment of the microbiota by the Ison-Hay method, to have a better diagnosis of BV, when the Nugent method is not used.  La vaginosis bacteriana (VB) es una alteración frecuente de la microbiota vaginal en mujeres en edad reproductiva. El diagnóstico puede ser efectuado aplicando criterios clínicos o por la evaluación de los morfotipos bacterianos presentes en la tinción de Gram realizada a la secreción vaginal o mediante procedimientos microbiológicos, los cuales se desarrollaron como una alternativa al diagnóstico clínico, reemplazándolo paulatinamente. El objetivo del presente trabajo fue determinar la efectividad de los métodos de Amsel e Ison-Hay, para el diagnóstico de vaginosis bacteriana, empleando el método de Nugent como estándar. En este estudio se analizaron 305 muestras de secreción vaginal de pacientes del Hospital Regional ISSSTE Puebla. Las muestras se procesaron y analizaron para el diagnóstico de VB, siguiendo las recomendaciones de los métodos de Amsel, Nugent e Ison-Hay. El análisis de los resultados indicó un 12.8 % por Nugent, 31.1 % de VB por el método de Amsel y 36.7 % por Ison-Hay; sugiriendo que ambas técnicas muestran una alta tasa de falsos positivos. La sensibilidad para el método de Amsel e Ison-Hay fue de 97.44 %, la especificidad fue de 78.57 % y 72.18 % para Amsel e Ison-Hay, respectivamente. En conclusión, dado a los resultados obtenidos y a las mínimas diferencias entre los métodos analizados, se recomienda realizar los criterios de Amsel, seguidos de la observación de la tinción de Gram del flujo vaginal para la valoración de la microbiota por el método de Ison-Hay, para tener un mejor diagnóstico de VB, cuando no se emplea el método de Nugent.  Universidad Autónoma de Tamaulipas 2020-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html text/xml https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1296 10.29059/cienciauat.v14i2.1296 CienciaUAT; Vol 14 No. 2. January-June 2020; 62-71 CienciaUAT; Vol. 14 No. 2: Enero-Junio 2020; 62-71 2007-7858 2007-7521 spa https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1296/667 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1296/669 https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/1296/769 Derechos de autor 2020 CienciaUAT

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