Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico

Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico

It is accepted that the presence of pathogen bacteria in food products made out of bovine is a human health risk because these microorganisms are capable of producing gastrointestinal diseases. This kind of pathology is the main cause of medical consultation and death in Mexico and the world. The pr...

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Main Authors: Cabrera-Maldonado, Carlos, León-Tello, Gloria, Tejeda-Trujillo, Fausto, Ramírez-Juárez, Belén, Flores-Encarnación, Marcos
Format: Online
Language:eng
Published: Universidad Autónoma de Tamaulipas 2013
Online Access:https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/11
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spelling oai:ojs.pkp.sfu.ca:article-112015-09-30T10:10:01Z Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico Estudio preliminar para investigar Salmonella sp. y E. Coli 0157:H7 en carne molida de res, de venta en supermercados en la ciudad de Puebla, México Cabrera-Maldonado, Carlos León-Tello, Gloria Tejeda-Trujillo, Fausto Ramírez-Juárez, Belén Flores-Encarnación, Marcos Ground beef gastrointestinal infections salmonellosis hemorrhagic colitis hemolytic uremic syndrome. carne molida infecciones gastrointestinales salmonelo­sis colitis hemorrágica síndrome urémico hemolítico It is accepted that the presence of pathogen bacteria in food products made out of bovine is a human health risk because these microorganisms are capable of producing gastrointestinal diseases. This kind of pathology is the main cause of medical consultation and death in Mexico and the world. The present study sought to find the presence of Salmonella sp. and Escherichia coli O157:H7 in 20 samples of ground beef, obtained at random in supermarkets in the city of Puebla, Mexico. The methodologies used were the ones described in the Mexican Official Standards: aerobic mesophilic bacteria (NOM-092-SSA1-1994) and Salmonella sp. (NOM-114-SSA1-1994), including the steps of pre-enrichment, selective enrichment, differential and enriched media inoculation, biochemical and serological identification. Even though neither Salmonella sp. nor E. coli O157:H7 strains were identified, the use of molecular techniques is recommended to make an exhaustive search of these pathogen agents, not only in ground beef, but also in other cattle-derived products.  Se ha reconocido que la presencia de bacterias patógenas en alimentos de origen bovino constituye un riesgo para la salud de las personas, debido a que estos microorganismos son capaces de generar problemas gastrointestinales, y son estas patologías una de las primeras causas de consulta médica y de muerte en México y en el mundo. El presente estudio buscó investigar la presencia de Salmonella sp. y Escherichia coli O157:H7 en 20 muestras de carne molida de res, adquiridas de manera aleatoria en supermercados de la ciudad de Puebla, Puebla, México. Las metodologías utilizadas fueron los procedimientos descritos en la Normas Oficiales Mexicanas: bacterias mesofílicas aerobias (NOM-092-SSA1-1994) y Salmonella sp. (NOM-114-SSA1-1994), que incluyeron las etapas de pre-enriquecimiento, enriquecimiento selectivo, inoculación en medios selectivos y diferenciales e identificación bioquímica y serológica. Aunque no se identificaron cepas de Salmonella sp. ni de E. coli O157:H7, se recomienda continuar con la búsqueda exhaustiva de estas bacterias o de otros agentes patógenos, no sólo en la carne molida de res, sino en otros productos derivados del ganado bovino, con la implementación de técnicas moleculares. Universidad Autónoma de Tamaulipas 2013-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/11 10.29059/cienciauat.v8i1.11 CienciaUAT; Vol. 8 No. 1: July-December 2013; 64-69 CienciaUAT; Vol. 8 No. 1: Julio-Diciembre 2013; 64-69 2007-7858 2007-7521 eng https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/11/104
institution CIENCIA UAT
collection OJS
language eng
format Online
author Cabrera-Maldonado, Carlos
León-Tello, Gloria
Tejeda-Trujillo, Fausto
Ramírez-Juárez, Belén
Flores-Encarnación, Marcos
spellingShingle Cabrera-Maldonado, Carlos
León-Tello, Gloria
Tejeda-Trujillo, Fausto
Ramírez-Juárez, Belén
Flores-Encarnación, Marcos
Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico
author_facet Cabrera-Maldonado, Carlos
León-Tello, Gloria
Tejeda-Trujillo, Fausto
Ramírez-Juárez, Belén
Flores-Encarnación, Marcos
author_sort Cabrera-Maldonado, Carlos
title Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico
title_short Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico
title_full Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico
title_fullStr Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico
title_full_unstemmed Preliminary study to investigate Salmonella sp. and E. Coli O157:H7 in ground beef sold in supermarkets in Puebla city, Mexico
title_sort preliminary study to investigate salmonella sp. and e. coli o157:h7 in ground beef sold in supermarkets in puebla city, mexico
description It is accepted that the presence of pathogen bacteria in food products made out of bovine is a human health risk because these microorganisms are capable of producing gastrointestinal diseases. This kind of pathology is the main cause of medical consultation and death in Mexico and the world. The present study sought to find the presence of Salmonella sp. and Escherichia coli O157:H7 in 20 samples of ground beef, obtained at random in supermarkets in the city of Puebla, Mexico. The methodologies used were the ones described in the Mexican Official Standards: aerobic mesophilic bacteria (NOM-092-SSA1-1994) and Salmonella sp. (NOM-114-SSA1-1994), including the steps of pre-enrichment, selective enrichment, differential and enriched media inoculation, biochemical and serological identification. Even though neither Salmonella sp. nor E. coli O157:H7 strains were identified, the use of molecular techniques is recommended to make an exhaustive search of these pathogen agents, not only in ground beef, but also in other cattle-derived products. 
publisher Universidad Autónoma de Tamaulipas
publishDate 2013
url https://revistaciencia.uat.edu.mx/index.php/CienciaUAT/article/view/11
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